In Vitro Studies on Aminoglycosides Permeation and Resorption in Porcine Skin as a Model Membrane

W. A. Awad‡,*1 and J. Zentek‡

*Clinic for Avian, Reptile and Fish Medicine, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine, A-1210 Vienna, Austria, ‡Institute of Animal Nutrition, Department of Veterinary Medicine, Freie Universität Berlin, 14195 Berlin, Germany

ABSTRACT

The in vitro study of drug skin permeability plays an essential role in the selection of drugs for the development of transdermal dosage forms. Therefore, the objective of present study was to state the dermal penetration, permeation and absorption of topically applied substances (Gentamicin and Polymyxin B sulphate). Skin samples of the ear and umbilical region of porcine were used in the experiments. At the beginning of the experiment, 500 µL of drug gel formulation was placed on the external side of the skin. After elapsed times of 30, 60, 90 and 120 min, 1.0 mL of the receiving solution was withdrawn and replaced with an equal volume of fresh buffer. The concentrations of each compound in the receiver medium were determined by high-performance liquid chromatography (HPLC). The results of the present study showed that all samples were under the detection limit. In conclusion, the systemic absorption for gentamicin and polymyxin B sulphate is poor when used topically.

Key words: antibiotics; gentamicin; polymyxin B sulphate; drug resorption; ototoxicity; porcine skin

Correspondence Author
wageha.awad@vetmeduni.ac.at